DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role...
23 KB (2,787 words) - 00:53, 18 June 2024
Ligation (molecular biology) (redirect from DNA ligation)
to create recombinant DNA molecules (such as when a foreign DNA fragment is inserted into a plasmid). The discovery of DNA ligase dates back to 1967 and...
36 KB (4,717 words) - 15:19, 12 September 2024
ligases include the word "ligase", such as in DNA ligase, an enzyme commonly used in molecular biology laboratories to join together DNA fragments. However,...
4 KB (430 words) - 15:26, 14 July 2024
DNA ligase 1 also DNA ligase I, is an enzyme that in humans is encoded by the LIG1 gene. DNA ligase 1 is the only known eukaryotic DNA ligase involved...
23 KB (2,878 words) - 08:09, 5 August 2024
DNA ligase 3 also DNA ligase III, is an enzyme that, in humans, is encoded by the LIG3 gene. LIG3 encodes ATP-dependent DNA ligases that seal interruptions...
30 KB (3,749 words) - 18:26, 16 August 2024
Okazaki fragments (category DNA replication)
discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication. They were discovered in the 1960s by...
33 KB (4,435 words) - 14:24, 7 June 2024
DNA ligase 4 also DNA ligase IV, is an enzyme that in humans is encoded by the LIG4 gene. DNA ligase 4 is an ATP-dependent DNA ligase that joins double-strand...
15 KB (1,821 words) - 05:44, 11 June 2024
capable of recognizing nicks in DNA. The ligase forms a DNA-adenylate complex, assisting recognition. With human DNA ligase, this forms a crystallized complex...
16 KB (2,041 words) - 14:01, 2 July 2024
Molecular cloning (redirect from Recombinant DNA technology)
enzyme, DNA ligase, fragments generated by restriction enzymes could be joined in new combinations, termed recombinant DNA. By recombining DNA segments...
32 KB (4,016 words) - 17:56, 20 August 2024
second component involves the bridging of DNA to DNA ligase 4, by hXRCC4, with the aid of Cernunnos-XLF. DNA-PKcs and hXRCC4 are anchored to Ku70 / Ku80...
43 KB (5,681 words) - 00:50, 14 June 2024
Natural competence (section Mechanisms of DNA uptake)
is the ability of a cell to alter its genetics by taking up extracellular DNA from its environment through a process called transformation. Competence...
17 KB (1,906 words) - 03:36, 5 August 2024
eukaryotes, newly synthesized lagging-strand DNA transiently contains nicks (before being sealed by DNA ligase) and provides a signal that directs mismatch...
35 KB (4,179 words) - 12:12, 19 July 2024
artificial DNA primers can be used to start DNA synthesis at known sequences in a template DNA molecule. Polymerase chain reaction (PCR), ligase chain reaction...
60 KB (7,286 words) - 08:26, 2 July 2024
joining (MMEJ), and homologous recombination (HR): In NHEJ, DNA Ligase IV, a specialized DNA ligase that forms a complex with the cofactor XRCC4, directly...
129 KB (15,900 words) - 06:12, 13 August 2024
Endonuclease (section DNA repair)
can be joined by DNA ligase. There are hundreds of restriction endonucleases known, each attacking a different restriction site. The DNA fragments cleaved...
25 KB (2,688 words) - 21:18, 6 April 2024
involvement of various polymerases, primers, and/or ligases. During the replication of DNA, for example, the DNA polymerase I leaves behind a hole between the...
5 KB (581 words) - 01:37, 14 August 2024
DNA ligase (NAD+) (EC 6.5.1.2, polydeoxyribonucleotide synthase (NAD+), polynucleotide ligase (NAD+), DNA repair enzyme, DNA joinase, polynucleotide synthetase...
2 KB (185 words) - 13:53, 26 August 2023
Nucleotide excision repair (category DNA repair)
Final ligation to complete NER and form a double stranded DNA is carried out by DNA ligase. NER can be divided into two subpathways: global genomic NER...
33 KB (3,623 words) - 18:02, 20 August 2024
Gene amplification (redirect from DNA amplification)
segment of DNA by polymerizing nucleotides, a concept which is applicable to numerous fields in modern biology and related sciences. Ligase chain reaction...
3 KB (345 words) - 00:43, 13 June 2020
Non-homologous end joining (category DNA repair)
XRCC4/DNA ligase IV complex and likely participates in the ligation step. Recent evidence suggests that XLF promotes re-adenylation of DNA ligase IV after...
28 KB (3,683 words) - 04:20, 22 August 2024
Artificial gene synthesis (redirect from DNA watermark)
oligonucleotide annealing, the synthesis step relies on a set of thermostable DNA ligase and polymerase enzymes. To date, several methods for gene synthesis have...
61 KB (7,073 words) - 16:39, 29 April 2024
E3 ligases regulates diverse areas such as cell trafficking, DNA repair, and signaling and is of profound importance in cell biology. E3 ligases are...
24 KB (2,754 words) - 17:41, 3 September 2024
and DNA fingerprinting. Enzymes called DNA ligases can rejoin cut or broken DNA strands. Ligases are particularly important in lagging strand DNA replication...
166 KB (17,865 words) - 10:19, 31 August 2024
Sequencing by ligation (redirect from DNA sequencing by ligation)
by ligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. Unlike...
4 KB (636 words) - 01:59, 11 December 2023
Base excision repair (redirect from Base excision DNA repair)
strand DNA replication. DNA ligase III along with its cofactor XRCC1 catalyzes the nick-sealing step in short-patch BER in humans. DNA ligase I ligates...
29 KB (3,493 words) - 00:14, 22 June 2024
endonucleases. At the replication fork, the gap in DNA after removal of the flap is sealed by DNA ligase I, which repairs the nicks that are left between...
119 KB (14,646 words) - 22:02, 3 July 2024
Gibson assembly (category DNA)
adjacent DNA fragments can anneal. The DNA polymerase incorporates nucleotides to fill in any gaps. The DNA ligase covalently joins the DNA of adjacent...
6 KB (689 words) - 15:42, 24 May 2024
gene. XRCC1 is involved in DNA repair, where it complexes with DNA ligase III. XRCC1 is involved in the efficient repair of DNA single-strand breaks formed...
23 KB (2,856 words) - 20:57, 6 March 2024
of a DNA strand with no attached complement) known as a sticky end on each end of AATT. The overhang can then be used to ligate in (see DNA ligase) a piece...
5 KB (629 words) - 16:22, 23 July 2024
Either DNA polymerase epsilon or DNA polymerase delta along with DNA ligase can be used to repair UV-damaged DNA. However, it is found that DNA polymerase...
3 KB (336 words) - 09:50, 4 November 2023