and ligand, or protein and nucleic acid binding interactions are frequently exploited for isolation of various biomolecules. Affinity chromatography is...

Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Like general chromatography...

radioligand binds with a single affinity. If the ligand were to have bound to multiple sites that have differing radioligand affinities, then the Scatchard plot...

can be purified by lectin affinity chromatography. Detergent-solubilized proteins can be allowed to bind to a chromatography resin that has been modified...

approaches. Affinity chromatography is performed following one of two basic formats: ligand immobilization or target immobilization. Under the ligand immobilization...

contaminants from protein samples. Affinity chromatography exploits the specific interactions between proteins and their ligands. A target protein is captured...

Thermofluor, which utilizes extrinsic fluorogenic dyes. The binding of low molecular weight ligands can increase the thermal stability of a protein, as...

(August 2017). "Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction". Cell Chemical Biology. 24 (8): 1040–1047...

proteins must have a high binding affinity when their ligand is present in high concentrations, but must also release the ligand when it is present at low concentrations...

Wulff, G.; Dederichs, R.; Grotstollen, R.; Jupe, C. (1982). "Affinity Chromatography and Related Techniques -Theoretical Aspects/Industrial and Biomedical...

used in affinity chromatography together with a column that has avidin (or streptavidin or neutravidin) bound to it, which is the natural ligand for biotin...

of selecting RNA ligands against T4 DNA polymerase and the lab of Jack Szostak, selecting RNA ligands against various organic dyes. Two years later,...

and engineering, particularly as an affinity material for sensors, detection of chemical, antimicrobial, and dye, residues in food, adsorbents for solid...

proteins is bound. Probe molecules, typically labeled with a fluorescent dye, are added to the array. Any reaction between the probe and the immobilised...

for the depressant effects of benzodiazepines. Flunitrazepam shows high affinity for the α-5 subunit of the GABA-A receptor, which causes some of its unique...

undergone relative to total protein concentration. By running an affinity chromatography, B-Galactosidase can be isolated and tested by reacting collected...

it is possible to incorporate a ligand to the target into one of the polymer phases. This improves the target's affinity to that phase, and improves its...

to assess purity; chromatography techniques are especially important for this application, and include HPLC and gas chromatography. Traditional methods...

high-performance liquid chromatography based measurements of lipophilicity to model biological distribution". Journal of Chromatography A. 1037 (1–2): 299–310...

Appel JR, Blanc P, Houghten RA (1993) Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries...

"Purification of Vicinal Dithiol-containing Proteins by Arsenical-Based Affinity Chromatography". In Sies, Helmut (ed.). Oxygen Radicals in Biological Systems...

C60, as well as other fullerenes. These fractions are separated using chromatography. Generally, the fullerenes are dissolved in a hydrocarbon or halogenated...

and receptor-ligand interactions. The SELDI process is a combination of surface-enhanced neat desorption (SEND),surface-enhanced affinity-capture (SEAC)...

spectrometry for particle mass, and particle counters for particle number. Chromatography, centrifugation, and filtration techniques can be used to separate nanoparticles...

drug recreationally. Detection in body fluids is typically by liquid chromatography-mass spectrometry. As of January 2018[update], neither the plant nor...

"Effect of the spacer arm on non-specific binding in membrane affinity chromatography". MRS Communications. 8 (1): 65–70. doi:10.1557/mrc.2018.4. S2CID 103064199...

down to +3 or +4. Secondly, a ligand is added to form a coordination complex. The ligand is chosen to have an affinity for the specific organ to be targeted...

exchange methods. The elements can also be separated by ion-exchange chromatography, making use of the fact that the stability constant for formation of...

various types of radiation. 1903 Mikhail Semyonovich Tsvet invents chromatography, an important analytic technique. 1904 Hantaro Nagaoka proposes an early...

analyzed using the tools of chemical analysis, e.g. spectroscopy and chromatography. Scientists engaged in chemical research are known as chemists. Most...