In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange...
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High-performance liquid chromatography (redirect from Gradient elution)
constant ("isocratic elution mode") or varied ("gradient elution mode") during the chromatographic analysis. Isocratic elution is typically effective...
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An antibody elution is a clinical laboratory diagnostic procedure which removes sensitized antibodies from red blood cells, in order to determine the...
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desorbed by an elution buffer. The mode used for elution (expanded-bed versus settled-bed) depends on the characteristics of the feed. After elution, the adsorbent...
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molecules, thus separate them. This type of elution is called gradient elution. On the other hand, step elution can be used, in which the concentration of...
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gel (stationary phase). To determine approximate molecular weight, the elution volumes of compounds with their corresponding molecular weights are obtained...
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pre-incubated drugs should mirror the elution profiles of the targets they complex with. Correlation of drug and protein elution profiles allows for targets to...
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elutes very late in the elution sequence, after fermium. Another method to isolate mendelevium exploits the distinct elution properties of Md2+ from those...
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bound. Target biomolecules may then be removed by applying a so-called elution buffer, which disrupts interactions between the bound target biomolecules...
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who in 1943 first classified the modes of chromatography as frontal, elution, and displacement. Displacement chromatography found a variety of applications...
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retained while impurities are washed away. An elution with a low pH buffer or a more gentle, high salt elution buffer is then used to recover purified antibody...
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known (see Mark–Houwink equation), a plot of log [η]M versus elution volume (or elution time) for a particular solvent, column and instrument provides...
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His-tag (section Elution method)
histidine and is typically used at a concentration of 150 - 500 mM for elution. Histidine or histamine can also be used. Decrease in pH When the pH decreases...
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Systematic evolution of ligands by exponential enrichment (section Binding sequence elution and amplification)
prepare for subsequent rounds of selection in which the stringency of the elution conditions can be increased to identify the tightest-binding sequences...
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divided into solvent selectivity groups. Using solvents with different elution strengths or different selectivity groups can often give very different...
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binding of biotinylated DNA to streptavidin-coated magnetic beads and elution of single DNA strands selectively using alkali. The method allowed robotic...
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the result of the first application of rate theory to the chromatography elution process. The van Deemter equation relates height equivalent to a theoretical...
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wool and silks, a colorant in food, drugs and cosmetics and an adsorption-elution indicator for chloride estimations in biochemistry. Tartrazine was discovered...
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partition coefficient from the partitioning experiment will indicate the elution behavior of the compound. Typically, it is desirable to choose a solvent...
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Elution: chromatographic separation of Fm(100), Es(99), Cf, Bk, Cm and Am...
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divalent state, unlike the other trivalent actinides: under typically used elution conditions (bis-(2-ethylhexyl) phosphoric acid (HDEHP) as stationary organic...
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X-ray fluorescence (XRF). Before the time that ion exchange methods and elution were available, the separation of the rare earths was primarily achieved...
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quantitation ions. VUV spectra can also be used to deconvolve analyte co-elution, resulting in an accurate quantitative representation of individual analyte...
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these materials and are afterwards desorbed either thermally (Tenax) or by elution (DNPH) and then analyzed by GC–MS/FID or HPLC. Reference gas mixtures are...
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by gradient elution. In MEKC, however, techniques must be used to extend the elution range to separate strongly retained analytes. Elution ranges can be...
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chromatography, the intrinsic viscosity of a polymer is directly related to the elution volume of the polymer. Therefore, by running several monodisperse samples...
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of 256Lr precluded a confirmation that it eluted ahead of Md3+ in the elution sequence. Lawrencium occurs as the trivalent Lr3+ ion in aqueous solution...
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incomplete recovery of the analytes by SPE caused by incomplete extraction or elution. In the case of an incomplete extraction, the analytes do not have enough...
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as a pharmaceutical is chemical, and the radionuclide is extracted by elution from germanium-68, a synthetic radioisotope of germanium, in gallium-68...
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particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in...
5 KB (639 words) - 04:32, 4 March 2024