isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. DNA extraction is the process of isolating DNA from the cells of an organism...
24 KB (3,168 words) - 15:17, 9 March 2024
Plasmid preparation (redirect from Dna preparation)
A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and...
17 KB (1,955 words) - 13:31, 10 June 2024
leaving only DNA. The most common methods of DNA extraction include organic extraction (also called phenol chloroform extraction), Chelex extraction, and solid...
104 KB (11,519 words) - 19:55, 5 May 2024
Plasmid (redirect from DNA plasmids)
plasmid DNA. Many commercial kits have been created to perform plasmid extraction at various scales, purity, and levels of automation. Plasmid DNA may appear...
46 KB (5,331 words) - 16:27, 6 May 2024
Archaeogenetics (category Ancient DNA (human))
contains six times the DNA when compared to stored bones. The temperature of extraction site also affects the amount of obtainable DNA, evident by a decrease...
47 KB (5,814 words) - 05:31, 17 May 2024
Cetrimonium bromide (section DNA extraction)
fungi. It is also one of the main components of some buffers for the extraction of DNA. It has been widely used in synthesis of gold nanoparticles (e.g....
21 KB (2,478 words) - 20:09, 7 April 2024
Body identification (section DNA extraction)
DNA identification are menstrual blood, venous blood, semen, saliva and vaginal secretion. The process of DNA profiling includes DNA extraction, DNA quantification...
26 KB (3,038 words) - 23:57, 27 November 2023
Electroelution (section DNA extraction)
phenol–chloroform extraction followed by ethanol precipitation. This method is simple, rapid and yields high recovery (75%) of DNA fragments from gel...
3 KB (299 words) - 09:09, 17 April 2024
Homo floresiensis (section DNA extraction attempt)
accounts for about 23% of Rampasasa villagers' complete DNA. That DNA also includes 0.8% of Denisovan DNA, which is more than that in populations from South-East...
71 KB (7,602 words) - 00:40, 26 June 2024
Library (biology) (redirect from DNA library)
different types of DNA libraries, including cDNA libraries (formed from reverse-transcribed RNA), genomic libraries (formed from genomic DNA) and randomized...
11 KB (1,204 words) - 21:21, 22 April 2024
of purifying their components, as in protein purification, DNA extraction, RNA extraction, or in purifying organelles. Many species of bacteria are subject...
10 KB (1,071 words) - 19:37, 18 February 2024
Protection of Underground Networks samples soil and extracts and sequences fungal DNA in order to learn which fungi are present. The geo-located fungal taxa are...
14 KB (1,242 words) - 02:33, 30 May 2024
Lysis buffer (category DNA)
macromolecules of the cells (e.g. western blot for protein, or for DNA extraction). Most lysis buffers contain buffering salts (e.g. Tris-HCl) and ionic...
15 KB (1,825 words) - 04:05, 23 December 2023
Forensic dentistry (section DNA extraction)
determinations by using radiographs, ante- and post-mortem photographs, and DNA analysis. Another type of evidence that may be analyzed is bite marks, whether...
71 KB (9,015 words) - 11:42, 12 May 2024
DNA fragmentation is the separation or breaking of DNA strands into pieces. It can be done intentionally by laboratory personnel or by cells, or can occur...
12 KB (1,498 words) - 02:31, 5 February 2024
more cellular debris prior to DNA extraction. Plasma is better than serum for ctDNA recovery The main appeal of ctDNA analysis is that it is extracted...
49 KB (5,856 words) - 05:57, 9 April 2024
or blood from e.g. leeches. DNA barcoding requires that DNA in the sample is extracted. Several different DNA extraction methods exist, and factors like...
99 KB (11,142 words) - 20:39, 26 May 2024
Genome skimming (category DNA sequencing methods)
low copy DNA. DNA extraction protocols will vary depending on the source of the sample (i.e. plants, animals, etc.). The following DNA extraction protocols...
42 KB (4,505 words) - 17:23, 26 January 2024
Differential extraction (also known as differential lysis) refers to the process by which the DNA from two different types of cells can be extracted without...
5 KB (761 words) - 11:15, 29 December 2021
any DNA sequencing protocol relies upon the DNA or RNA sample extraction and preparation from the biological material of interest. A successful DNA extraction...
129 KB (14,413 words) - 10:39, 30 May 2024
Column purification DNA extraction Ethanol precipitation Phenol-chloroform extraction Peirson SN, Butler JN (2007). "RNA extraction from mammalian tissues"...
6 KB (680 words) - 17:42, 5 July 2023
detection of rare tumour-derived DNA (ctDNA) from blood samples. DNA extraction Ethanol precipitation Phenol-chloroform extraction Spin column-based purification...
14 KB (2,152 words) - 00:40, 11 March 2023
cancer). Circulating tumor DNA (ctDNA) refers to DNA released by cancerous cells into the blood stream. Cancer mutations in ctDNA mirror those found in traditional...
25 KB (2,235 words) - 18:20, 19 April 2024
Extraction of DNA sample (sometimes repeated if the first sample proves too degraded for analysis) Fundraising for DNA sequencing Sequencing of DNA sample...
190 KB (21,057 words) - 01:36, 26 June 2024
specifically for each type of extraction. For DNA extraction, the pH is adjusted to 7.0–8.0. For RNA-specific extraction, the pH is adjusted to 4.5. At...
4 KB (470 words) - 20:13, 3 October 2023
Ethanol precipitation (section DNA precipitation)
to the tube when using isopropanol. DNA extraction Phenol–chloroform extraction Salting in Salting out SCODA DNA purification Spin column-based nucleic...
8 KB (1,039 words) - 16:48, 28 March 2020
Liquid–liquid extraction, also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative...
46 KB (5,792 words) - 01:51, 18 June 2024
complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both...
19 KB (2,275 words) - 13:01, 9 May 2024
protonates the iminodiacetate groups. Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including...
2 KB (283 words) - 14:04, 28 March 2023
Surveyor nuclease assay (section DNA Extraction)
Initially, the DNA of interest (nuclear or mitochondrial DNA) is extracted from tissues or cell culture. This can be done by standard extraction methods such...
26 KB (3,176 words) - 03:46, 22 January 2024