dates back to 1967 and was an important event in the field of molecular biology. Ligation in the laboratory is normally performed using T4 DNA ligase....
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Directionality, in molecular biology and biochemistry, is the end-to-end chemical orientation of a single strand of nucleic acid. In a single strand of...
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Look up ligation in Wiktionary, the free dictionary. Ligation may refer to: Ligation (molecular biology), the covalent linking of two ends of DNA or RNA...
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solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase...
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In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...
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Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication...
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In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant...
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In Molecular biology, an insert is a piece of DNA that is inserted into a larger DNA vector by a recombinant DNA technique, such as ligation or recombination...
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Multiplex ligation-dependent probe amplification (MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple...
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Dawson PE (March 2019). "Native Chemical Ligation of Peptides and Proteins". Current Protocols in Chemical Biology. 11 (1): e61. doi:10.1002/cpch.61. PMC 6384150...
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cellular and molecular biology is a list of definitions of terms and concepts commonly used in the study of cell biology, molecular biology, and related...
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blunt ends, but blunt-end ligation is inefficient and requires a higher concentration of both DNA and DNA ligase than the ligation of sticky ends does. For...
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biology have emerged from studies of RNA (ribonucleic acid), including seminal work in the fields of biochemistry, genetics, microbiology, molecular biology...
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Subcloning (category Molecular biology)
In molecular biology, subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination vector. Subcloning is not...
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preparation with SAFE Hi-C uses ligation products for deep sequencing to improve traditional Hi-C analysis". Communications Biology. 2 (1): 267. doi:10.1038/s42003-019-0519-y...
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Ligase (redirect from Enzymatic ligation)
In biochemistry, a ligase is an enzyme that can catalyze the joining (ligation) of two molecules by forming a new chemical bond. This is typically via...
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Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Research applications)...
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Genetics (redirect from Genetic biology)
sequence of DNA—a phenomenon Francis Crick called the central dogma of molecular biology. The specific sequence of amino acids results in a unique three-dimensional...
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"Lecture 20: Gel Electrophoresis". BCH5425 Molecular Biology and Biotechnology. Bowen, R (20 October 1999). "DNA Ligation". Biotechnology and Genetic Engineering...
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in plants List of geneticists Human Genome Project Dot plot (bioinformatics) Multiplex Ligation-dependent Probe Amplification Sequence analysis v t e...
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was the first enzyme isolated from this strain.[citation needed] In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky...
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Cross-linking immunoprecipitation (redirect from CLIP (molecular biology))
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to...
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single assay. The design of the molecular inversion probes (MIP) originated from padlock probes, a molecular biology technique first reported by Nilsson...
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Blue–white screen (section Molecular mechanism)
vector) grow into blue colonies. Molecular cloning is one of the most commonly used procedures in molecular biology. A gene of interest may be inserted...
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Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras". Next Generation Sequencing. Methods in Molecular Biology. Vol. 1712. pp. 71–85...
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DNA sequencing (category Molecular biology)
KP (2012). "Palindromic sequence impedes sequencing-by-ligation mechanism". BMC Systems Biology. 6 (Suppl 2): S10. doi:10.1186/1752-0509-6-S2-S10. PMC 3521181...
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NdeI (section Use in molecular biology)
NdeI is an endonuclease isolated from Neisseria denitrificans. In molecular biology, it is commonly used as a restriction enzyme. Recognition sequence...
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DNA shuffling (section Molecular breeding)
sequences for restriction enzyme recognition sites which are needed for ligation into a cloning vector. It is possible to recombine portions of the parent...
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exonuclease and annealing step while the other is for DNA polymerase and ligation steps. For the two-step approach, different incubation temperatures are...
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Protein splicing (redirect from Expressed protein ligation)
segment (called an intein) is removed from a precursor protein with a ligation of C-terminal and N-terminal external proteins (called exteins) on both...
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