CRISPR activation (CRISPRa) is a gene regulation technique that utilizes an engineered form of the CRISPR-Cas9 system to enhance the expression of specific...
22 KB (2,739 words) - 07:27, 29 September 2024
debate. CRISPR activation Anti-CRISPR CRISPR/Cas Tools CRISPR gene editing The CRISPR Journal "Designer baby" DRACO Gene knockout Genome-wide CRISPR-Cas9...
128 KB (13,448 words) - 11:44, 28 September 2024
CRISPR gene editing (CRISPR, pronounced /ˈkrɪspər/ "crisper", refers to "clustered regularly interspaced short palindromic repeats") is a genetic engineering...
170 KB (19,897 words) - 03:44, 24 September 2024
Cas9 (redirect from CRISPR/Cas9)
Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense...
43 KB (5,609 words) - 10:59, 18 September 2024
Anti-CRISPR (Anti-Clustered Regularly Interspaced Short Palindromic Repeats or Acr) is a group of proteins found in phages, that inhibit the normal activity...
32 KB (3,691 words) - 06:48, 24 August 2024
Sequence-specific activation of gene expression refers to CRISPR activation (CRISPRa). Based on the bacterial genetic immune system - CRISPR (clustered regularly...
24 KB (2,898 words) - 12:08, 22 March 2024
In molecular biology, trans-activating CRISPR RNA (tracrRNA) is a small trans-encoded RNA. It was first discovered by Emmanuelle Charpentier in her study...
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CRISPR RNA or crRNA is a RNA transcript from the CRISPR locus. CRISPR-Cas (clustered, regularly interspaced short palindromic repeats - CRISPR associated...
10 KB (1,149 words) - 06:02, 17 June 2024
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
55 KB (6,164 words) - 23:47, 3 December 2023
CRISPR-Display (CRISP-Disp) is a modification of the CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) system for genome editing...
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RNA polymerase that recruits RNA polymerase to the lac promoter. CRISPR activation Bacterial transcription Coactivator (genetics) Eukaryotic transcription...
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breaks requiring activation of host repair mechanisms. They also lack Cas1 and Cas2 proteins and so rely on other more complete CRISPR systems for spacer...
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infection, CTL activation is tightly controlled and in general requires a very strong MHC/antigen activation signal, or additional activation signals provided...
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editing with engineered nucleases. Alongside zinc finger nucleases and CRISPR/Cas9, TALEN is a prominent tool in the field of genome editing. TAL effectors...
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Guide RNA (section CRISPR In Prokaryotes)
used for gene editing. In bacteria and archaea, gRNAs are a part of the CRISPR-Cas system that serves as an adaptive immune defense that protects the organism...
30 KB (3,628 words) - 15:29, 5 August 2024
Designer baby (section CRISPR/Cas9)
homology directed repair. The CRISPR/Cas9 system (CRISPR – Clustered Regularly Interspaced Short Palindromic Repeats, Cas9 – CRISPR-associated protein 9) is...
71 KB (8,294 words) - 16:50, 27 September 2024
Epigenome editing (section CRISPR-Cas)
Zinc finger proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps...
30 KB (3,420 words) - 09:03, 16 August 2024
Genome editing (section CRISPR)
transcription activator-like effector nucleases (TALENs) and engineered meganucleases—were selected by Nature Methods as the 2011 Method of the Year. The CRISPR-Cas...
78 KB (9,361 words) - 01:20, 25 September 2024
S phases in the cell cycle. CRISPR gene editing based on Clustered regularly interspaced short palindromic repeats (CRISPR) -Cas9 is an enzyme that uses...
11 KB (1,360 words) - 02:06, 3 December 2023
on pooled genetic perturbation screens. Perturb-seq combines multiplexed CRISPR mediated gene inactivations with single cell RNA sequencing to assess comprehensive...
21 KB (2,588 words) - 18:53, 19 April 2024
clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9, transcription activator-like effector nucleases (TALEN), meganucleases, and zinc...
53 KB (6,846 words) - 19:05, 8 July 2024
Human genetic enhancement (section CRISPR/Cas9)
benefits of revolutionary tools like CRISPR are endless. For example, because it can be applied directly in the embryo, CRISPR/Cas9 reduces the time required...
96 KB (10,514 words) - 21:07, 2 October 2024
Gene knockdown (section CRISPRs)
or CRISPRs. CRISPR-associated (cas) genes encode cellular machinery that cuts exogenous DNA into small fragments and inserts them into a CRISPR repeat...
13 KB (1,611 words) - 09:30, 4 May 2023
Germline mutation (section CRISPR/Cas9 editing)
initiates cellular repair processes, similar to that of CRISPR/Cas9 DNA editing. Compared to CRISPR/Cas9, the therapeutic applications of this technology...
25 KB (3,085 words) - 03:46, 17 September 2024
Gene knockout (section CRISPR/Cas9)
done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs. One of the main advantages of gene knockouts is that they...
21 KB (2,524 words) - 18:03, 29 April 2024
brother used a CRISPR transcriptional activator which functions to upregulate an alternative isoform of dystrophin. Because the CRISPR activation technology...
7 KB (730 words) - 18:56, 25 September 2023
NMD are significant when designing CRISPR-Cas9 experiments, particularly those aimed at gene inactivation. CRISPR-Cas9 introduces double-strand breaks...
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been developed. Transcription activator-like effector nucleases (TALENs) and the Cas9-guideRNA system (adapted from CRISPR) are the two most common. Many...
55 KB (6,765 words) - 09:23, 25 May 2024
Locus Biosciences (section CRISPR CAS3)
develops phage therapies based on CRISPR–Cas3 gene editing technology, as opposed to the more commonly used CRISPR-Cas9, delivered by engineered bacteriophages...
18 KB (1,451 words) - 10:30, 28 February 2024
ISSN 0002-9289. PMID 1781479. The-Crispr (2019-05-13). "Antidote to deadly jellyfish identified using CRISPR". The Crispr. Retrieved 2019-08-08. Neely, G...
8 KB (523 words) - 11:51, 18 May 2024