Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense...
43 KB (5,610 words) - 21:17, 2 May 2024
CRISPR gene editing (redirect from CRISPR-Cas9 gene editing)
on a simplified version of the bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA)...
170 KB (19,936 words) - 16:29, 8 August 2024
CRISPR (redirect from CRISPR/Cas9-mediated genome editing)
complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes...
128 KB (13,448 words) - 03:26, 19 August 2024
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
55 KB (6,164 words) - 23:47, 3 December 2023
Guide RNA (section CRISPR Cas9)
RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9-endonuclease or other Cas-proteins that cut the double-stranded DNA and thereby...
30 KB (3,628 words) - 15:29, 5 August 2024
Epigenome editing (redirect from FIRE-Cas9)
proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps with gene expression...
30 KB (3,420 words) - 09:03, 16 August 2024
CRISPR activation (section dCas9)
on Cas9 (from Type II systems), but other effectors like Cas12a (Type V) have been used as well. Cas9 Endonuclease Dead, also known as dead Cas9 or dCas9...
21 KB (2,672 words) - 09:24, 16 August 2024
along with the CRISPR-Cas9 machinery. When the modified organism mates and its DNA mixes with that of its mate, the CRISPR-Cas9 tool cuts the partner's...
47 KB (5,127 words) - 10:04, 8 June 2024
Cas12a (section Cas9 vs. Cas12a)
highly targeted modifications of DNA, similar to CRISPR-Cas9 system. Cas12a is distinguished from Cas9 by a its single RuvC endonuclease active site, its 5'...
13 KB (1,530 words) - 21:01, 18 August 2024
CRISPR-associated nuclease such as Cas9 attaches to a tracrRNA–crRNA complex, which guides Cas9 to the invading protospacer sequence. But Cas9 will not cleave the protospacer...
12 KB (1,421 words) - 00:22, 14 December 2022
Off-target genome editing (section Cas9 nickases)
as: clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9, transcription activator-like effector nucleases (TALEN), meganucleases,...
53 KB (6,846 words) - 19:05, 8 July 2024
Francisco in early 2013. The technology uses a catalytically dead Cas9 (usually denoted as dCas9) protein that lacks endonuclease activity to regulate genes...
24 KB (2,898 words) - 12:08, 22 March 2024
CRISPR–Cas9, the type of mechanism used in the genetic edition of human cells). A year later, a study confirmed the presence of type II-A CRISPR–Cas9 inhibitors...
32 KB (3,691 words) - 06:48, 24 August 2024
contains regularly spaced CRISPR/Cas9 target sites into the genomes of progenitor cells. Alongside the barcode, Cas9 and sgRNA are introduced into the...
22 KB (2,706 words) - 21:31, 14 August 2024
modification of the CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) system for genome editing. The CRISPR/Cas9 system uses a short guide...
16 KB (2,133 words) - 12:04, 22 March 2024
marker for plant research to improve gene editing methods such as CRISPR/Cas9. In varying degrees most mammals have some skin areas without natural hair...
5 KB (531 words) - 06:00, 2 June 2024
Doudna and Emmanuelle Charpentier were the first to propose that CRISPR-Cas9 (enzymes from bacteria that control microbial immunity) could be used for...
68 KB (5,876 words) - 17:52, 7 August 2024
such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), are also improving the nutrition, taste and yield of crops. There are many...
32 KB (3,576 words) - 19:10, 19 August 2024
Designer baby (section CRISPR/Cas9)
homology directed repair. The CRISPR/Cas9 system (CRISPR – Clustered Regularly Interspaced Short Palindromic Repeats, Cas9 – CRISPR-associated protein 9) is...
71 KB (8,210 words) - 04:47, 10 July 2024
Human germline engineering (section CRISPR-Cas9)
2015, a group of Chinese scientists used the gene-editing technique CRISPR/Cas9 to edit single-celled, non-viable embryos to see the effectiveness of this...
54 KB (6,767 words) - 11:14, 17 July 2024
(January 2017). "Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA". Journal...
15 KB (1,247 words) - 20:27, 12 May 2024
DNA site. It uses a fusion protein, consisting of a catalytically impaired Cas9 endonuclease fused to an engineered reverse transcriptase enzyme, and a prime...
26 KB (3,050 words) - 04:06, 9 August 2024
Germline mutation (section CRISPR/Cas9 editing)
protein Cas9 to break the DNA backbones at specific target sequences. This system has shown a higher specificity than TALENs or ZFNs due to the Cas9 protein...
25 KB (3,085 words) - 14:19, 5 August 2024
Gene knockout (section CRISPR/Cas9)
through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs. One of the main advantages of gene knockouts is that they allow...
21 KB (2,524 words) - 18:03, 29 April 2024
Antibiotic (section CRISPR-Cas9-based treatments)
invading viruses. The system, known as CRISPR-Cas9, consists of (a) an enzyme that destroys DNA (the nuclease Cas9) and (b) the DNA sequences of previously...
155 KB (14,572 words) - 17:36, 19 August 2024
Human genetic enhancement (section CRISPR/Cas9)
endless. For example, because it can be applied directly in the embryo, CRISPR/Cas9 reduces the time required to modify target genes compared to gene targeting...
95 KB (10,491 words) - 00:01, 9 August 2024
S, et al. (December 2015). "Generation of gene-target dogs using CRISPR/Cas9 system". Journal of Molecular Cell Biology. 7 (6): 580–3. doi:10.1093/jmcb/mjv061...
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are significant when designing CRISPR-Cas9 experiments, particularly those aimed at gene inactivation. CRISPR-Cas9 introduces double-strand breaks that...
23 KB (2,900 words) - 08:39, 17 August 2024
No-SCAR genome editing (redirect from No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing)
donor DNA integration while Cas9 cleaves double-stranded DNA to counter-select against wild-type cells. Although λ-Red and Cas9 genome editing are widely...
44 KB (5,650 words) - 01:08, 30 July 2024
Emmanuelle Charpentier (section CRISPR/Cas9)
laboratory showed that Cas9 could be used to make cuts in any DNA sequence desired. The method they developed involved the combination of Cas9 with easily created...
43 KB (3,302 words) - 14:30, 18 July 2024