Hybridoma technology is a method for producing large numbers of identical antibodies, also called monoclonal antibodies. This process starts by injecting...

Hybridoma Bank (DSHB) is a National Resource established by the National Institutes of Health (NIH) in 1986 to bank and distribute at cost hybridomas...

human-derived hybridomas. In 1975, Georges Köhler and César Milstein succeeded in making fusions of myeloma cell lines with B cells to create hybridomas that could...

medicine and research. In contrast to monoclonal antibodies produced by hybridoma technology, which may lose the capacity to produce the desired antibody...

antibodies isolated from cell culture supernatants of these hybridoma clones (hybridoma lines) are simply called monoclonals. Monoclonal neoplasm (tumor):...

A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell (myeloma). The rabbit immune...

1984 with Niels Kaj Jerne and Georges J. F. Köhler for developing the hybridoma technique for the production of monoclonal antibodies. Milstein was born...

Biology field Bioreactor Cell culture Cultured meat Flow cytometry Hybridoma technology HPLC NMR Spectroscopy Chemical field Centrifugation CSTR Crystallization...

César Milstein and Georges Köhler reported their discovery of how to use hybridoma cells to isolate monoclonal antibodies, effectively beginning the history...

hypoxic conditions as found in pathology such as myocardial ischemia. Hybridomas are immortal (immune to cellular senescence), HGPRT+ cells that result...

named 9E10, is available from the non-commercial Developmental Studies Hybridoma Bank. Protein tag Polyhistidine-tag SpyTag Flag-tag Evan GI, Lewis GK...

Lymphocytes and Two Distinct Monocyte Subsets in Human Peripheral Blood". Hybridoma. 7 (6): 521–527. doi:10.1089/hyb.1988.7.521. PMID 2466760. Passlick, Bernward;...

the experimental fusion of two genetically different cells, as e.g., in hybridoma technology. Heterokaryon is from neo-classic Greek hetero, meaning different...

line. The fused cells are called hybridomas, and will continually grow and secrete antibody in culture. Single hybridoma cells are isolated by dilution...

(GRMFT): a unique lymphokine made by normal T lymphocytes and a T cell hybridoma". Journal of Immunology. 132 (2): 821–7. doi:10.4049/jimmunol.132.2.821...

created directly from subcloned heavy and light chains derived from a hybridoma. ScFvs have many uses, e.g., flow cytometry, immunohistochemistry, and...

monoclonal antibody technology. Soll directed the Developmental Studies Hybridoma Bank from 1995 to 2021, and the WM Keck Dynamic Image Analysis Facility...

required for the action of many antiviral drugs. It is used to select hybridoma cell lines in production of monoclonal antibodies. In clinical chemistry...

Biology field Bioreactor Cell culture Cultured meat Flow cytometry Hybridoma technology HPLC NMR Spectroscopy Chemical field Centrifugation CSTR Crystallization...

can be fused with myeloma to produce an immortal line of cells called hybridoma that secrete immunoglobin. Hybrid cell lines are also used to study cancer...

degradation of chromosome ends during DNA replication in eukaryotes. Hybridoma technology, specifically used for generating immortalised antibody-producing...

combined with an immortal myeloma cell line (B cell lineage) to produce a hybridoma which has the antibody specificity of the primary lymphocyte and the immortality...

following the discovery of the structure of antibodies and the development of hybridoma technology, which provided the first reliable source of monoclonal antibodies...

formulation called Rhoclone (Bharat Serums and Vaccines Ltd.), made from hybridoma cultures. The country has also tested a recombinant version of Rhoclone...

oxygen uptake and lactate production rates. With the introduction of hybridoma technology in 1975, cell culture could be applied towards the generation...

antibodies, antihemophilic factors, vaccines and many other drugs. Mouse hybridomas, cells fused together to create monoclonal antibodies, have been adapted...

fight off bacteria and viruses, but they are "custom-designed" (using hybridoma technology or other methods) and can therefore be made specifically to...

used for preparation of monoclonal antibodies. This process is called hybridoma technology. Laboratory animals (e.g., mice) are first exposed to an antigen...

by Rouvier et al. who isolated IL17A transcript from a rodent T-cell hybridoma. The protein encoded by IL17A is a founding member of IL-17 family (see...

Biology field Bioreactor Cell culture Cultured meat Flow cytometry Hybridoma technology HPLC NMR Spectroscopy Chemical field Centrifugation CSTR Crystallization...