In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds...

Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal...

Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses...

current nuclease-based gene editing platforms but its use was limited by low efficiencies of editing. Genome editing with engineered nucleases, i.e. all...

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger...

Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample...

Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for...

Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels). Surveyor...

Serratia marcescens nuclease (EC 3.1.30.2, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme....

Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made...

MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure...

helices α4 and α5 and two loops P1 and P2 of the cleavage domain. When the nuclease is unbound to DNA, the endonuclease domain is sequestered by the DNA-binding...

Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a...

Mung bean nuclease (Nuclease MB) is a nuclease derived from sprouts of the mung bean (Vigna radiata) that removes nucleotides in a step-wise manner from...

DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. The PAM is a component...

for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications...

finger nucleases. Since 2009 more accurate and easier systems to implement have been developed. Transcription activator-like effector nucleases (TALENs)...

CRISPR-associated systems, cas 1–4. The Cas proteins showed helicase and nuclease motifs, suggesting a role in the dynamic structure of the CRISPR loci....

foods. Cellulases, pectinases Clarify fruit juices. Molecular biology Nucleases, DNA ligase and polymerases Use restriction digestion and the polymerase...

are most accessible. Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds. Nucleases that hydrolyse nucleotides...

enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. Evidence...

be generated by fusing a natural or engineered DNA-binding domain to a nuclease domain (often the cleavage domain of the type IIS restriction enzyme FokI)...

a result another nuclease must be recruited to cleave the flap. This second nuclease is DNA2 nuclease, which has a helicase-nuclease activity, that cleaves...

Information, U.S. National Library of Medicine. "Entrez Gene: APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1". Parker A, Gu Y, Mahoney W, Lee...

CUT&RUN sequencing, also known as cleavage under targets and release using nuclease, is a method used to analyze protein interactions with DNA. CUT&RUN sequencing...

response, increasing the efficiency of prime editing. Nuclease Prime Editor uses Cas9 nuclease instead of Cas9(H840A) nickase. Unlike prime editor 3 (PE3)...

Cas9 contains two nuclease domains: an McrA-like HNH nuclease domain and a RuvC-like nuclease domain. These HNH and RuvC-like nuclease domains are responsible...

2007).. A broad range of enzymes including DNA ligase, DNA polymerase and nucleases have been employed to generate high-fidelity SNP genotyping methods. Restriction...

carbohydrates such as starch and sugars into simple sugars such as glucose, and nucleases split nucleic acids into nucleotides. Digestive enzymes are found throughout...

in lysosomes, which are acidic on the inside. Acid hydrolases may be nucleases, proteases, glycosidases, lipases, phosphatases, sulfatases and phospholipases...