Polymerase chain reaction (redirect from PCR oil)
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...
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2021. "Actualización de la situación epidemiológica de las variantes de importancia en salud pública en España (18 de marzo de 2021)" [Update of the...
219 KB (18,220 words) - 21:11, 3 January 2025
Real-time polymerase chain reaction (redirect from Real-Time PCR)
(real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors...
43 KB (5,346 words) - 00:33, 23 December 2024
Digital polymerase chain reaction (redirect from Digital PCR)
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction...
69 KB (7,351 words) - 04:46, 30 October 2024
from the very beginning of November where the PCR tests had S gene target failure (occurs in a few variants, but not in Delta which dominated in the country...
219 KB (24,993 words) - 15:39, 24 December 2024
monoclonal antibodies. PCR tests continue to detect the Omicron variant. The WHO defines a previously circulating variant as a variant that "has demonstrated...
225 KB (21,654 words) - 04:28, 5 January 2025
COVID-19 testing (redirect from PCR testing procedure)
as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used. The Minimum...
181 KB (21,007 words) - 05:52, 6 January 2025
Microsatellite (section ISSR-PCR)
to determine the genotype and variants at repetitive loci. Microsatellites can be analysed and verified by established PCR amplification and amplicon size...
67 KB (7,394 words) - 16:46, 2 September 2024
is based on SSP-PCR, it is possible that new variants, in particular in the class I and DRB1 loci, may be missed. For example, SSP-PCR within the clinical...
51 KB (5,942 words) - 07:28, 11 November 2024
beyond that of real-time PCR. Point mutations, loss of heterozygosity and aneuploidy can be detected in a single PCR step. Digital PCR can differentiate between...
51 KB (5,955 words) - 06:39, 30 December 2024
be amplified by PCR. PCR amplifies all probe pairs that have been successfully ligated, using fluorescently labeled PCR primers. The PCR products are quantified...
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Ștefan Foriș (section PCR outlawing)
journalist who served as general secretary of the Romanian Communist Party (PCR or PCdR) between December 1940 and April 1944. Born a Transylvanian Csángó...
107 KB (14,515 words) - 13:44, 3 January 2025
in the Tirol region, where nearly 9% of the positive PCR tests were identified as the Beta variant by sequencing. The active cases were estimated at around...
72 KB (6,070 words) - 20:49, 10 September 2024
Single-nucleotide polymorphism (redirect from Single nucleotide variant)
cancer cells. DNA variants must also commonly be taken into consideration in molecular diagnostics applications such as designing PCR primers to detect...
58 KB (6,252 words) - 04:35, 6 January 2025
ARMS-PCR method was developed for the simultaneous detection of C677T and A1298C polymorphisms with the A66G MTRR polymorphism in a single PCR reaction...
35 KB (3,743 words) - 21:22, 25 December 2024
Circulating free DNA (section PCR)
PCR amplification of mutated genes from extracted cfDNA. PCR based analysis of cfDNA typically rely on the analytical nature of qPCR and digital PCR....
37 KB (4,210 words) - 04:41, 30 December 2024
VZV by PCR". J. Med. Virol. 54 (3): 155–157. doi:10.1002/(SICI)1096-9071(199803)54:3<155::AID-JMV1>3.0.CO;2-4. PMID 9515761. S2CID 24215093. De Paschale...
80 KB (8,861 words) - 13:41, 3 January 2025
Canine parvovirus (section Variants)
by either an ELISA or a hemagglutination test, or by electron microscopy. PCR has become available to diagnose CPV2, and can be used later in the disease...
30 KB (3,495 words) - 19:34, 21 December 2024
Mutagenesis (molecular biology technique) (redirect from Error-prone PCR)
oligonucleotide synthesis, or conducting a PCR reaction in conditions that enhance misincorporation of nucleotides (error-prone PCR), for example by reducing the fidelity...
25 KB (2,895 words) - 04:30, 1 January 2025
neutralisation. Newer methods use enzyme immunoassays (EIA). In the years before PCR was invented immunofluorescence was used to quickly confirm viral infections...
58 KB (7,208 words) - 21:01, 17 November 2024
Marburg virus disease (redirect from Variant U)
not feasible options. The most common diagnostic methods are therefore RT-PCR in conjunction with antigen-capture ELISA, which can be performed in field...
86 KB (7,522 words) - 08:02, 15 December 2024
Hi-C (genomic analysis technique) (section Variants)
PCR artifacts (such as off-target amplicons, non-specificity, etc.). The ideal range of PCR cycles is 9–15 and it is more ideal to pool multiple PCR reactions...
98 KB (10,972 words) - 12:56, 11 June 2024
PMC 11527365. PMID 39480750. Jackson CJ, Mochizuki T, Barton RC (2006). "PCR fingerprinting of Trichophyton mentagrophytes var. interdigitale using polymorphic...
11 KB (1,138 words) - 11:15, 2 January 2025
2022–2023 mpox outbreak (category CS1 German-language sources (de))
2022. "Salud informa que el resultado de la muestra PCR tomada por ANLIS Malbrán al primer caso sospechoso de viruela símica dio positivo" (in Spanish)...
186 KB (20,425 words) - 19:47, 30 December 2024
CZ 75 (section Development of sport variants of CZ 75)
There is a version available chambered for the .40 S&W cartridge. CZ 75 D PČR Compact Very compact – similar to the P-01 in size, with an aluminum alloy...
41 KB (4,360 words) - 12:36, 1 January 2025
expression for production of the relevant carbapenamase. Nested arbitrary PCR (ARB-PCR) was used during a 2007 CRE outbreak at the University of Virginia Medical...
69 KB (8,479 words) - 19:42, 20 October 2024
Systematic evolution of ligands by exponential enrichment (section SELEX variants and alternative aptamer selection methods)
capture on paramagnetic beads. The bound sequences are eluted and amplified by PCR to prepare for subsequent rounds of selection in which the stringency of...
41 KB (4,747 words) - 15:29, 2 November 2024
Soviet spies, together with whom he joined the Romanian Communist Party (PCR). He expressed his loyalty toward Gheorghe Gheorghiu-Dej, the jailed communist...
87 KB (11,337 words) - 14:40, 3 January 2025
COVID-19 (section SARS-CoV-2 variants)
acid include real-time reverse transcription polymerase chain reaction (RT‑PCR), transcription-mediated amplification, and reverse transcription loop-mediated...
288 KB (33,190 words) - 07:03, 5 January 2025