Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a...
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quantifying ANAs are indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA). In immunofluorescence, the level of autoantibodies is...
54 KB (6,097 words) - 21:43, 28 June 2024
diagnosed with the aid of immunofluorescence studies. Cutaneous conditions with positive direct or indirect immunofluorescence when using salt-split skin...
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Direct fluorescent antibody (redirect from Direct immunofluorescence)
A direct fluorescent antibody (DFA or dFA), also known as "direct immunofluorescence", is an antibody that has been tagged in a direct fluorescent antibody...
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Anti-dsDNA antibodies (section Immunofluorescence)
Blood tests such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence are routinely performed to detect anti-dsDNA antibodies in diagnostic...
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Pemphigoid (section Direct immunofluorescence)
histopathology and direct immunofluorescence, indirect immunofluorescence and the ELISA test. Among all, direct immunofluorescence is the gold standard for...
50 KB (5,853 words) - 14:30, 2 December 2024
specialized testing needs to be performed on biopsies, including immunofluorescence, immunohistochemistry, electron microscopy, flow cytometry, and molecular-pathologic...
51 KB (5,585 words) - 21:51, 7 December 2024
Main antinuclear antibody patterns on immunofluorescence. CREST syndrome typically displays the centrome pattern....
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Immunofluorescence pattern of SS-A and SS-B antibodies. Produced using serum from a patient on HEp-20-10 cells with a FITC conjugate....
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crescents will be normally observed. When these are subjected to immunofluorescence, three patterns can be observed: linear, granular and negative (pauci-immune)...
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Immunofluorescence micrograph of three cytotoxic T cells (outer three) surrounding a cancer cell. Lytic granules (red) are secreted at the contact site...
14 KB (1,377 words) - 16:49, 20 November 2024
immunohistochemistry, or when the stain is a fluorescent molecule, immunofluorescence. This technique has greatly increased the ability to identify categories...
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severity of portal hypertension. Main antinuclear antibody patterns on immunofluorescence A schematic representation of an antibody JB Imboden, DB Hellmann...
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non-organ- and non-species-specific anti-organelle antibody detected by immunofluorescence: the mitochondrial antibody number 5. Clin Exp Immunol 31(3):357-366...
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become the primary diagnostic tool in the future.[citation needed] Immunofluorescence or immunoperoxidase assays are commonly used to detect whether a virus...
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direct immunofluorescence microscopy (DIF) on a skin biopsy specimen, and (3) positive epidermal side staining by indirect immunofluorescence microscopy...
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assistant divides the specimen(s) for submission for light microscopy, immunofluorescence microscopy and electron microscopy. The pathologist will examine the...
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Fluorescence microscope (section Immunofluorescence)
different molecule which binds the target of interest within the sample. Immunofluorescence is a technique which uses the highly specific binding of an antibody...
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cerebellum with bound anti-GAD65 monoclonal antibodies (green), indirect immunofluorescence Specialty Neurology Symptoms muscular rigidity and trigger-induced...
27 KB (2,962 words) - 23:32, 7 December 2024
done via antibody staining, hemadsorption using red blood cells, or immunofluorescence microscopy. Shell vial cultures, which can identify infection via...
114 KB (13,194 words) - 06:46, 4 December 2024
Lupus band test is done upon skin biopsy, with direct immunofluorescence staining, in which, if positive, IgG and complement depositions are found at the...
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Three-Dimensional Imaging with Confocal Laser Scanning Microscopy and Double Immunofluorescence". Modern Pathology. 14 (9): 854–861. doi:10.1038/modpathol.3880401...
39 KB (3,009 words) - 18:03, 30 November 2024
Adenoviral keratoconjunctivitis is done using cell culture (with immunofluorescence staining) and PCR. It may appear similar to herpes simplex type I...
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Immunofluorescence image of HeLa cells grown in tissue culture and stained with antibody to actin in green, vimentin in red and DNA in blue...
49 KB (5,688 words) - 22:51, 13 December 2024
basement membrane without a hyperproliferation of the glomerular cells. Immunofluorescence demonstrates diffuse granular uptake of IgG. The basement membrane...
21 KB (2,134 words) - 13:51, 22 September 2024
the skin; lesion) Direct immunofluorescence showing intercellular and basement membrane staining Indirect immunofluorescence staining with rat bladder...
21 KB (2,526 words) - 21:24, 12 August 2023
Pemphigus Acantholytic cells with direct immunofluorescence positivity With the use of direct immunofluorescence examination, the specificity of cytology...
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Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941. This led to the later development of immunohistochemistry...
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include northern hybridisation, quantitative RT-PCR, Western blot, immunofluorescence, ELISA and phenotypic analysis. The new genetic material can be inserted...
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systemic vasculitis, so called ANCA-associated vasculitides (AAV). Immunofluorescence (IF) on ethanol-fixed neutrophils is used to detect ANCA, although...
20 KB (2,272 words) - 06:48, 24 August 2024