Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I-like mechanism to carry out site...
15 KB (1,745 words) - 12:21, 16 August 2024
Recombinases are genetic recombination enzymes. DNA recombinases are widely used in multicellular organisms to manipulate the structure of genomes, and...
7 KB (753 words) - 23:57, 27 November 2023
Cre-Lox recombination (section Cre recombinase)
Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites...
40 KB (5,058 words) - 07:42, 8 May 2024
FLP-FRT recombination (redirect from FLP recombinase)
sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 μ plasmid of baker's yeast Saccharomyces...
21 KB (2,655 words) - 10:31, 15 May 2024
Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). By adding a reverse transcriptase...
14 KB (1,704 words) - 18:00, 3 December 2023
Site-specific recombinase technologies are genome engineering tools that depend on recombinase enzymes to replace targeted sections of DNA. In the late...
26 KB (3,200 words) - 20:41, 26 March 2024
V(D)J recombination (redirect from VDJ recombinase)
majority of T cells. The process of V(D)J recombination is mediated by VDJ recombinase, which is a diverse collection of enzymes. The key enzymes involved are...
21 KB (2,860 words) - 07:32, 14 March 2024
certain degree of sequence homology. Enzymes known as site-specific recombinases (SSRs) perform rearrangements of DNA segments by recognizing and binding...
19 KB (1,982 words) - 17:01, 30 March 2024
Hin recombinase is a 21kD protein composed of 198 amino acids that is found in the bacteria Salmonella. Hin belongs to the serine recombinase family (B2)...
4 KB (525 words) - 01:39, 14 June 2024
DNA integration, by means of site-specific recombinase technology, performed by a specific class of recombinase enzymes ("integrases") Economic integration...
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recombination. Recombination between LoxP sites is catalysed by Cre recombinase. The term "floxing" is a portmanteau constructed from the phrase "flanking/flanked...
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Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells. Through selective mutation, Cre recombinase...
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RMCE (recombinase-mediated cassette exchange) is a procedure in reverse genetics allowing the systematic, repeated modification of higher eukaryotic genomes...
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RAD51 (redirect from Rad51 recombinase)
RadB and RadC recombinase paralogs are found in many organisms belonging to Euryarchaeota, while a broader diversity of related recombinase paralogs are...
48 KB (5,526 words) - 16:55, 15 July 2024
abnormalities. The recombination reaction is catalyzed by enzymes known as recombinases, such as RAD51. The first step in recombination is a double-stranded...
166 KB (17,865 words) - 08:43, 15 August 2024
enzymes involved in spermatogenesis, spermatogenic DNA polymerase beta and recombinase activities evolving a unique temperature optimum that is slightly less...
61 KB (6,237 words) - 09:17, 5 August 2024
recombination (HR) ordinarily occurs during meiosis. The meiosis-specific recombinase, Dmc1, is required for efficient meiotic HR, and Dmc1 is expressed in...
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meiosis-specific recombinase Dmc1 and the general recombinase Rad51 coat the single-stranded DNA to form nucleoprotein filaments. The recombinases catalyze invasion...
32 KB (3,709 words) - 22:10, 25 July 2024
with an aspartic acid residue in order to disable the activity of the recombinases and inactivate homologous recombination. The endA1 mutation inactivates...
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engineering. These genetically engineered mouse models typically use a Cre recombinase system to disrupt tumor suppressors or activate oncogenes specifically...
76 KB (8,857 words) - 08:48, 12 August 2024
commonly used technique is the Cre-lox recombination system. The Cre recombinase enzyme specifically recognizes two lox (loci of recombination) sites...
9 KB (1,292 words) - 05:52, 19 December 2023
that enable transgenic expression of FRT recombination sites and a Flp recombinase in non-human mammals. Her technology has aided many researchers in achieving...
21 KB (2,397 words) - 05:05, 19 July 2024
body temperature in most of mammals, as β-polimerase and spermatogenic recombinase need a specific optimal temperature. Consequently, the testes are located...
33 KB (3,731 words) - 17:27, 15 August 2024
proteins of the RecA recombinase family of proteins are thought to be descended from a common ancestral recombinase. The RecA recombinase family contains RecA...
106 KB (12,113 words) - 01:28, 14 August 2024
Recombination in bacteria is ordinarily catalyzed by a RecA type of recombinase. These recombinases promote repair of DNA damages by homologous recombination....
12 KB (1,283 words) - 02:59, 29 January 2024
recombinase called Cre, the original conditional knockout technique recombined short target sequences known as LoxP. Since then, other recombinases have...
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FtsK (section Recombinase (Xer D) activation)
family of tyrosine recombinases. The interaction of Xer D and the γ subunit of FtsKC results in the activation of the recombinase. Contact between Xer...
12 KB (1,519 words) - 00:35, 29 November 2023
as psm-mecRNA. SCCmec also contains ccrA and ccrB; both genes encode recombinases that mediate the site-specific integration and excision of the SCCmec...
106 KB (12,066 words) - 17:53, 20 August 2024
station based in Biloxi, Mississippi Cre-Lox recombination a site-specific recombinase technology widely used to carry out deletions, insertions, translocations...
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different enzymes. Recombinases are key enzymes that catalyse the strand transfer step during recombination. RecA, the chief recombinase found in Escherichia...
33 KB (3,952 words) - 03:09, 12 August 2024