In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange...
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An antibody elution is a clinical laboratory diagnostic procedure which removes sensitized antibodies from red blood cells, in order to determine the...
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High-performance liquid chromatography (redirect from Gradient elution)
constant ("isocratic elution mode") or varied ("gradient elution mode") during the chromatographic analysis. Isocratic elution is typically effective...
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desorbed by an elution buffer. The mode used for elution (expanded-bed versus settled-bed) depends on the characteristics of the feed. After elution, the adsorbent...
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molecules, thus separate them. This type of elution is called gradient elution. On the other hand, step elution can be used, in which the concentration of...
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gel (stationary phase). To determine approximate molecular weight, the elution volumes of compounds with their corresponding molecular weights are obtained...
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pre-incubated drugs should mirror the elution profiles of the targets they complex with. Correlation of drug and protein elution profiles allows for targets to...
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elutes very late in the elution sequence, after fermium. Another method to isolate mendelevium exploits the distinct elution properties of Md2+ from those...
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bound. Target biomolecules may then be removed by applying a so-called elution buffer, which disrupts interactions between the bound target biomolecules...
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His-tag (section Elution method)
histidine and is typically used at a concentration of 150 - 500 mM for elution. Histidine or histamine can also be used. Decrease in pH When the pH decreases...
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known (see Mark–Houwink equation), a plot of log [η]M versus elution volume (or elution time) for a particular solvent, column and instrument provides...
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who in 1943 first classified the modes of chromatography as frontal, elution, and displacement. Displacement chromatography found a variety of applications...
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retained while impurities are washed away. An elution with a low pH buffer or a more gentle, high salt elution buffer is then used to recover purified antibody...
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the result of the first application of rate theory to the chromatography elution process. The van Deemter equation relates height equivalent to a theoretical...
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divided into solvent selectivity groups. Using solvents with different elution strengths or different selectivity groups can often give very different...
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Systematic evolution of ligands by exponential enrichment (section Binding sequence elution and amplification)
prepare for subsequent rounds of selection in which the stringency of the elution conditions can be increased to identify the tightest-binding sequences...
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chromatogram. The separate curves in the diagram represent different sample elution concentration profiles over time based on their affinity to the column...
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Elution: chromatographic separation of Fm(100), Es(99), Cf, Bk, Cm and Am...
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binding of biotinylated DNA to streptavidin-coated magnetic beads and elution of single DNA strands selectively using alkali. The method allowed robotic...
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wool and silks, a colorant in food, drugs and cosmetics and an adsorption-elution indicator for chloride estimations in biochemistry. Tartrazine was discovered...
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chromatography, the intrinsic viscosity of a polymer is directly related to the elution volume of the polymer. Therefore, by running several monodisperse samples...
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divalent state, unlike the other trivalent actinides: under typically used elution conditions (bis-(2-ethylhexyl) phosphoric acid (HDEHP) as stationary organic...
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these materials and are afterwards desorbed either thermally (Tenax) or by elution (DNPH) and then analyzed by GC–MS/FID or HPLC. Reference gas mixtures are...
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X-ray fluorescence (XRF). Before the time that ion exchange methods and elution were available, the separation of the rare earths was primarily achieved...
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partition coefficient from the partitioning experiment will indicate the elution behavior of the compound. Typically, it is desirable to choose a solvent...
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as a pharmaceutical is chemical, and the radionuclide is extracted by elution from germanium-68, a synthetic radioisotope of germanium, in gallium-68...
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changed to elute the purified nucleic acid off the column using a suitable elution buffer. The basic principle of the phenol-chloroform extraction is that...
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emitter. 68Ga is a positron emitter, with a 68-minute half-life, produced by elution from germanium-68 in a gallium-68 generator or by proton irradiation of...
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incomplete recovery of the analytes by SPE caused by incomplete extraction or elution. In the case of an incomplete extraction, the analytes do not have enough...
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within two daughter half-lives. Hence, removing the daughter nuclide (elution process) from the generator ("milking" the cow) is reasonably done as often...
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