• The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying fluorescence microscopy. Experimental...
    5 KB (582 words) - 01:21, 2 August 2020
  • Thumbnail for Green fluorescent protein
    fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers...
    63 KB (7,449 words) - 09:33, 14 July 2024
  • the photoprotein aequorin and green fluorescent protein (GFP) and for his proposal to use GFP as a tracer molecule. He communicated his pioneering work...
    19 KB (2,069 words) - 00:25, 17 October 2024
  • Thumbnail for DNA replication
    budding yeast by monitoring green fluorescent protein (GFP)-tagged DNA polymerases α. They detected DNA replication of pairs of the tagged loci spaced apart...
    60 KB (7,286 words) - 08:26, 2 July 2024
  • Thumbnail for PGLO
    araC, a promoter region that regulates the expression of GFP (specifically, the GFP gene will be expressed only in the presence of arabinose) GFP, the...
    6 KB (612 words) - 23:00, 23 October 2023
  • Thumbnail for RoGFP
    fluorescent protein (roGFP) is a green fluorescent protein engineered to be sensitive to changes in the local redox environment. roGFPs are used as redox-sensitive...
    12 KB (1,214 words) - 01:30, 3 December 2023
  • Thumbnail for Transcription (biology)
    transcriptase synthesises a complementary strand of DNA to form a double helix DNA structure (cDNA). The cDNA is integrated into the host cell's genome by the...
    58 KB (6,788 words) - 18:55, 25 October 2024
  • Thumbnail for RNA-directed DNA methylation
    GFP-expressing plant, and triggering silencing of GFP. The same study showed that a subset of these mobile sRNAs were triggering the addition of DNA methylation...
    139 KB (14,822 words) - 02:25, 16 July 2024
  • Thumbnail for Fluorescence microscope
    detection of normal blinking fluorescent dyes like green fluorescent protein (GFP) can be achieved by using a further development of SPDM the so-called SPDMphymod...
    25 KB (2,729 words) - 15:18, 24 October 2024
  • Thumbnail for DNA-functionalized quantum dots
    fluorescent protein (GFP) was used to track movement inside cells. However, GFP does not light up well and is unstable after application. Thus, GFP prevented long...
    18 KB (2,315 words) - 00:12, 10 June 2024
  • Thumbnail for Fluorophore
    larger natural fluorophores that are proteins: green fluorescent protein (GFP) is 27 kDa, and several phycobiliproteins (PE, APC...) are ≈240kDa. As of...
    29 KB (2,272 words) - 16:42, 3 November 2024
  • (August 1999). "A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii". The Plant Journal...
    61 KB (7,073 words) - 16:39, 29 April 2024
  • Thumbnail for Intracellular pH
    pH-sensitive GFPs are often used as part of a noninvasive and effective technique. By using cDNA as a template along with the appropriate primers, the GFP gene...
    17 KB (1,891 words) - 19:58, 16 May 2024
  • Thumbnail for DNA shuffling
    Additionally, the improvement of green fluorescent protein (GFP) was accomplished with DNA shuffling by molecular breeding as a 45-fold greater signal...
    25 KB (2,913 words) - 04:36, 21 September 2023
  • Thumbnail for Caenorhabditis elegans
    that DNA repair capability underlies longevity. The capacity to repair DNA damage by the process of nucleotide excision repair declines with age. C. elegans...
    93 KB (10,603 words) - 21:11, 29 October 2024
  • Thumbnail for Mosaic (genetics)
    Mosaic (genetics) (redirect from Mosaic DNA)
    express GFP in specific cells. However, a globally expressed GAL80 gene is used to repress the action of GAL4, preventing the expression of GFP. Instead...
    23 KB (2,639 words) - 10:06, 31 October 2024
  • Thumbnail for Dronpa
    acids long and is a 28.8 kDa monomer. Dronpa is 76% similar in sequence to GFP and shares a similar structure with an 11 stranded β-barrel (a β-can) enclosing...
    6 KB (619 words) - 12:18, 27 August 2021
  • acid (e.g. a nsyb-QF2, QUAS-GFP, tub-QS fly expresses no GFP when its diet doesn't contain quinic acid, and expresses GFP in its neurons when fed quinic...
    12 KB (1,520 words) - 11:35, 31 December 2023
  • Thumbnail for Branchiostoma floridae
    ; Ma, L.; Mushegian, A.; Sanders, E.; Schwartz, J.; Ron Yu, C. (2008). "A family of GFP-like proteins with different spectral properties in lancelet...
    6 KB (578 words) - 15:56, 13 September 2024
  • Thumbnail for Genetic transformation
    ones. Other commonly used reporter genes are green fluorescent protein (GFP), which produces cells that glow green under blue light, and the enzyme luciferase...
    54 KB (6,762 words) - 07:39, 2 November 2024
  • Thumbnail for Bioreporter
    bioreporters, the signal must be activated by an external light source (GFP and UMT), and for a select few bioreporters, the signal is completely self-induced...
    14 KB (1,843 words) - 05:47, 1 May 2024
  • therapeutic proteins). Fluorescence microscopy: Fluorescent markers such as GFP, are used to label a specific component of the cell. Afterwards, a certain...
    41 KB (5,252 words) - 05:01, 2 November 2024
  • any other DNA element, and is used as a probe to hybridize a cDNA, cRNA or genomic DNA sample (called a target) under high-stringency conditions. Probe-target...
    315 KB (29,380 words) - 23:23, 22 October 2024
  • protein localisation HomoloGene, compares proteins from different species gfp-cdna, protein localisation with fluorescence microscopy International Protein...
    7 KB (621 words) - 20:49, 21 June 2024
  • Thumbnail for Immunofluorescence
    containing fluorescent protein domains, e.g., green fluorescent protein (GFP). The GFP-technique involves altering the genetic information of the cells. A...
    21 KB (2,054 words) - 16:47, 21 April 2024
  • Thumbnail for Chimera (genetics)
    S2CID 30883689. Rakosy-Tican, E.; Aurori, C.M.; Dijkstra, C.; Thieme, R.; Aurori, A. & Davey, M.R. (2007). "The usefulness of the gfp reporter gene for monitoring...
    57 KB (6,610 words) - 23:10, 29 October 2024
  • Thumbnail for Fluorescent tag
    fluorescent proteins or FPs were introduced. Green fluorescent protein or GFP was discovered by Osamu Shimomura in the 1960s and was developed as a tracer...
    24 KB (2,782 words) - 17:34, 31 October 2024
  • Thumbnail for Systematic evolution of ligands by exponential enrichment
    uses of the technique are suggested by aptamers that bind tumor markers, GFP-related fluorophores, and a VEGF-binding aptamer trade-named Macugen has...
    41 KB (4,747 words) - 15:29, 2 November 2024
  • Thumbnail for Cell cycle
    transcription in yeast Cell Cycle Animation 1Lec.com Cell Cycle Fucci:Using GFP to visualize the cell-cycle Science Creative Quarterly's overview of the...
    78 KB (9,091 words) - 19:45, 9 October 2024
  • Thumbnail for GAL4/UAS system
    GFP. In the offspring, the desired subset of cells will make GAL4, and in these cells the GAL4 will bind to the UAS, and enable the production of GFP...
    13 KB (1,800 words) - 00:46, 20 September 2023