The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying fluorescence microscopy. Experimental...
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Green fluorescent protein (redirect from GFP tag)
fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers...
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the photoprotein aequorin and green fluorescent protein (GFP) and for his proposal to use GFP as a tracer molecule. He communicated his pioneering work...
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budding yeast by monitoring green fluorescent protein (GFP)-tagged DNA polymerases α. They detected DNA replication of pairs of the tagged loci spaced apart...
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PGLO (section Discovery of GFP)
araC, a promoter region that regulates the expression of GFP (specifically, the GFP gene will be expressed only in the presence of arabinose) GFP, the...
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fluorescent protein (roGFP) is a green fluorescent protein engineered to be sensitive to changes in the local redox environment. roGFPs are used as redox-sensitive...
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Transcription (biology) (redirect from DNA transcription)
transcriptase synthesises a complementary strand of DNA to form a double helix DNA structure (cDNA). The cDNA is integrated into the host cell's genome by the...
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GFP-expressing plant, and triggering silencing of GFP. The same study showed that a subset of these mobile sRNAs were triggering the addition of DNA methylation...
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detection of normal blinking fluorescent dyes like green fluorescent protein (GFP) can be achieved by using a further development of SPDM the so-called SPDMphymod...
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fluorescent protein (GFP) was used to track movement inside cells. However, GFP does not light up well and is unstable after application. Thus, GFP prevented long...
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larger natural fluorophores that are proteins: green fluorescent protein (GFP) is 27 kDa, and several phycobiliproteins (PE, APC...) are ≈240kDa. As of...
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Artificial gene synthesis (redirect from DNA watermark)
(August 1999). "A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii". The Plant Journal...
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Intracellular pH (section pH-sensitive GFPs)
pH-sensitive GFPs are often used as part of a noninvasive and effective technique. By using cDNA as a template along with the appropriate primers, the GFP gene...
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Additionally, the improvement of green fluorescent protein (GFP) was accomplished with DNA shuffling by molecular breeding as a 45-fold greater signal...
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Caenorhabditis elegans (redirect from C elegans)
that DNA repair capability underlies longevity. The capacity to repair DNA damage by the process of nucleotide excision repair declines with age. C. elegans...
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Mosaic (genetics) (redirect from Mosaic DNA)
express GFP in specific cells. However, a globally expressed GAL80 gene is used to repress the action of GAL4, preventing the expression of GFP. Instead...
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acids long and is a 28.8 kDa monomer. Dronpa is 76% similar in sequence to GFP and shares a similar structure with an 11 stranded β-barrel (a β-can) enclosing...
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acid (e.g. a nsyb-QF2, QUAS-GFP, tub-QS fly expresses no GFP when its diet doesn't contain quinic acid, and expresses GFP in its neurons when fed quinic...
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; Ma, L.; Mushegian, A.; Sanders, E.; Schwartz, J.; Ron Yu, C. (2008). "A family of GFP-like proteins with different spectral properties in lancelet...
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Genetic transformation (redirect from DNA transfer)
ones. Other commonly used reporter genes are green fluorescent protein (GFP), which produces cells that glow green under blue light, and the enzyme luciferase...
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Bioreporter (section Green fluorescent protein (GFP))
bioreporters, the signal must be activated by an external light source (GFP and UMT), and for a select few bioreporters, the signal is completely self-induced...
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therapeutic proteins). Fluorescence microscopy: Fluorescent markers such as GFP, are used to label a specific component of the cell. Afterwards, a certain...
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any other DNA element, and is used as a probe to hybridize a cDNA, cRNA or genomic DNA sample (called a target) under high-stringency conditions. Probe-target...
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protein localisation HomoloGene, compares proteins from different species gfp-cdna, protein localisation with fluorescence microscopy International Protein...
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containing fluorescent protein domains, e.g., green fluorescent protein (GFP). The GFP-technique involves altering the genetic information of the cells. A...
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Chimera (genetics) (redirect from Chimaera (DNA))
S2CID 30883689. Rakosy-Tican, E.; Aurori, C.M.; Dijkstra, C.; Thieme, R.; Aurori, A. & Davey, M.R. (2007). "The usefulness of the gfp reporter gene for monitoring...
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fluorescent proteins or FPs were introduced. Green fluorescent protein or GFP was discovered by Osamu Shimomura in the 1960s and was developed as a tracer...
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uses of the technique are suggested by aptamers that bind tumor markers, GFP-related fluorophores, and a VEGF-binding aptamer trade-named Macugen has...
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transcription in yeast Cell Cycle Animation 1Lec.com Cell Cycle Fucci:Using GFP to visualize the cell-cycle Science Creative Quarterly's overview of the...
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GFP. In the offspring, the desired subset of cells will make GAL4, and in these cells the GAL4 will bind to the UAS, and enable the production of GFP...
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