Dental pulp stem cell

Dental pulp stem cells (DPSCs) are stem cells present in the dental pulp, which is the soft living tissue within teeth. DPSCs can be collected from dental pulp by means of a non-invasive practice. It can be performed with an adult after simple extraction or to the young after surgical extraction of wisdom teeth.[1] They are pluripotent, as they can form embryoid body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice.[2] DPSCs can differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers.[2] They can differentiate into many cell types, such as odontoblasts, neural progenitors, osteoblasts, chondrocytes, and adipocytes. DPSCs were found to be able to differentiate into adipocytes and neural-like cells.[3] DPSC differentiation into osteogenic lines is enhanced in 3D condition and hypoxia.[4] These cells can be obtained from postnatal teeth, wisdom teeth, and deciduous teeth, providing researchers with a non-invasive method of extracting stem cells.[5] The different cell populations, however, differ in certain aspects of their growth rate in culture, marker gene expression and cell differentiation, although the extent to which these differences can be attributed to tissue of origin, function or culture conditions remains unclear.[6] As a result, DPSCs have been thought of as an extremely promising source of cells used in endogenous tissue engineering.[7]

Studies have shown that the proliferation rate of DPSCs is 30% higher than in other stem cells, such as bone marrow stromal stem cells (BMSSCs).[8] These characteristics of DPSCs are mainly due to the fact that they exhibit elevated amounts of cell cycling molecules, one being cyclin-dependent kinase 6 (CDK6), present in the dental pulp tissue.[8] Additionally, DPSCs have displayed lower immunogenicity than MSCs.[9]

Atari et al., established a protocol for isolating and identifying the subpopulations of dental pulp pluripotent-like stem cells (DPPSC). These cells are SSEA4+, OCT3/4+, NANOG+, SOX2+, LIN28+, CD13+, CD105+, CD34-, CD45-, CD90+, CD29+, CD73+, STRO1+, and CD146-, and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique.[2]

Role in regenerative dentistry

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The human mouth is vulnerable to craniofacial defects, microbial attacks, and traumatic damages.[10] Although preclinical and clinical partial regeneration of dental tissues has shown success, the creation of an entire tooth from DPSCs is not yet possible.[10]

Distraction osteogenesis

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Distraction osteogenesis (DO) is a method of bone regeneration, commonly used in the surgical repair of large craniofacial defects.[7] The area in which the defect is present is purposely broken in surgery, allowed to heal briefly, and then the bone segments are gradually separated until the area has healed satisfactorily. A study conducted in 2018 by Song et al. found that DPSCs transfected with Sirtuin-1 (SIRT1) in rabbits were more effective in promoting bone formation during DO.[7] SIRT1 directly regulated MSCs into osteoblasts which then shows the accumulation of significantly higher levels of calcium after osteogenic differentiation in vitro, suggesting the potential role of DPSCs in enhancing the efficiency of DO.[7]

Calcined tooth powder

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Calcine tooth powder (CTP) is obtained by burning extracted teeth, destroying the potential infection-causing material within the tooth, resulting in tooth ash [11] Tooth ash has been shown to promote bone repair.[12] Although recent studies have shown that calcine tooth powder- culture media (CTP-CM) does not affect proliferation, they have shown that CTP-CM has significantly increased levels of osteo/odontogenic markers in DPSCs.[11]

Stem cells from human exfoliated deciduous teeth

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Stem cells from human exfoliated deciduous teeth (SHED) are similar to DPSCs in the sense that they are both derived from the dental pulp, but SHED are derived from baby teeth, whereas DPSCs are derived from adult teeth. SHED are a population of multipotent stem cells that are easily collected, as deciduous teeth either shed naturally or are physically removed in order to facilitate the proper growth of permanent teeth.[13][14] These cells can differentiate into osteocytes, adipocytes, odontoblast, and chondrocytes in vitro.[14] Recent work has shown the enhanced proliferative capabilities of SHED when compared with that of dental pulp stem cells.[14]

Potential therapeutic use of SHED

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Studies have shown that under the influence of oxidative stress, SHED (OST-SHED) displayed increased levels of neuronal protection.[15] The properties of these cells exhibited in this study suggest that OST-SHED could potentially prevent of oxidative stress-induced brain damage and could aid in the development of therapeutic tools for neurodegenerative disorders.[15] After SHED injection into Goto-Kakizaki rats, type II diabetes mellitus (T2DM) was ameliorated, suggesting the potential for SHED in T2DM therapies.[16]

Recent studies have also shown that the administration of SHED in mice ameliorated the T cell immune imbalance in allergic rhinitis (AR), suggesting the cells' potential in future AR treatments.[17] After introducing SHED, mice experienced reduced nasal symptoms and decreased inflammatory infiltration.[17] SHEDs were found to inhibit the proliferation of T lymphocytes, increase levels of an anti-inflammatory cytokine, IL-10, and decrease the levels of a pro-inflammatory cytokine, IL-4.[17]

Additionally, SHED can potentially treat liver cirrhosis.[18] In a study conducted by Yokoyama et al. (2019), SHED were differentiated into hepatic stellate cells.[18] They found that when hepatic cells derived from SHED were transplanted into the liver of rats, liver fibrosis was terminated, allowing for the healing of the liver structure.[18]

History

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  • In 2000, a population of odontogenic progenitor cells with high self-renewal and proliferative capacity was identified in the dental pulp of humans permanent third molars.[19]
  • 2005 NIH announces discovery of DPSCs by Dr. Irina Kerkis [20]
  • 2006 IDPSC Kerkis reported discovery of Immature Dental Pulp Stem Cells (IDPSC),[21] a pluripotent sub-population of DPSC using dental pulp organ culture.
  • 2007 DPSC 1st animal studies begin for bone regeneration.[22][23]
  • 2007 DPSC 1st animal studies begin for dental end uses.[24][25]
  • 2008 DPSC 1st animal studies begin for heart therapies.[26]
  • 2008 IDPSC 1st animal study began for muscular dystrophy therapies.[27]
  • 2008 DPSC 1st animal studies begin for regenerating brain tissue.[28][failed verification]
  • 2008 DPSC 1st advanced animal study for bone grafting announced. Reconstruction of large size cranial bone defects in rats.[29]
  • 2010 IDPSC 1st human trial for cornea replacement

[30]

References

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  1. ^ Huang, G. T.; Gronthos, S.; Shi, S. (2009). "Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources". Journal of Dental Research. 88 (9): 792–806. doi:10.1177/0022034509340867. PMC 2830488. PMID 19767575.
  2. ^ a b c Atari, M.; Gil-Recio, C.; Fabregat, M.; García-Fernández, D.; Barajas, M.; Carrasco, M. A.; Jung, H. S.; Alfaro, F. H.; Casals, N.; Prosper, F.; Ferrés-Padró, E.; Giner, L. (2012). "Dental pulp of the third molar: A new source of pluripotent-like stem cells". Journal of Cell Science. 125 (Pt 14): 3343–56. doi:10.1242/jcs.096537. PMID 22467856.
  3. ^ Gronthos, S.; Brahim, J.; Li, W.; Fisher, L. W.; Cherman, N.; Boyde, A.; Denbesten, P.; Robey, P. G.; Shi, S. (2002). "Stem cell properties of human dental pulp stem cells". Journal of Dental Research. 81 (8): 531–5. doi:10.1177/154405910208100806. PMID 12147742. S2CID 8921170.
  4. ^ Labedz-Maslowska, A.; Bryniarska, N.; Kubiak, A.; Kaczmarzyk, T.; Sekula-Stryjewska, M.; Noga, S.; Boruczkowski, D.; Madeja, Z.; Zuba-Surma, E. (2020). "Multilineage Differentiation Potential of Human Dental Pulp Stem Cells-Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro". Int J Mol Sci. 21 (17): 6172. doi:10.3390/ijms21176172. PMC 7504399. PMID 32859105.
  5. ^ Morsczeck, C.; Reichert, T. E. (2018). "Dental stem cells in tooth regeneration and repair in the future". Expert Opinion on Biological Therapy. 18 (2): 187–196. doi:10.1080/14712598.2018.1402004. PMID 29110535. S2CID 41147569.
  6. ^ Volponi, A. A. (2010). "Stem cell-based biological tooth repair and regeneration". Trends in Cell Biology. 20 (12): 715–722. doi:10.1016/j.tcb.2010.09.012. PMC 3000521. PMID 21035344.
  7. ^ a b c d Song, D.; Xu, P.; Liu, S.; Wu, S. (2019). "Dental pulp stem cells expressing SIRT1 improve new bone formation during distraction osteogenesis". American Journal of Translational Research. 11 (2): 832–843. PMC 6413255. PMID 30899383.
  8. ^ a b Shi, S.; Robey, P. G.; Gronthos, S. (2001). "Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis". Bone. 29 (6): 532–9. doi:10.1016/S8756-3282(01)00612-3. PMID 11728923.
  9. ^ Ching, H. S.; Luddin, N.; Rahman, I. A.; Ponnuraj, K. T. (2017). "Expression of Odontogenic and Osteogenic Markers in DPSCS and SHED: A Review". Current Stem Cell Research & Therapy. 12 (1): 71–79. doi:10.2174/1574888X11666160815095733. PMID 27527527.
  10. ^ a b Amrollahi, P.; Shah, B.; Seifi, A.; Tayebi, L. (2016). "Recent advancements in regenerative dentistry: A review". Materials Science and Engineering: C. 69: 1383–90. doi:10.1016/j.msec.2016.08.045. PMID 27612840.
  11. ^ a b Wu, J.; Li, N.; Fan, Y.; Wang, Y.; Gu, Y.; Li, Z.; Pan, Y.; Romila, G.; Zhou, Z.; Yu, J. (2019). "The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways". Stem Cells International. 2019: 4793518. doi:10.1155/2019/4793518. PMC 6444228. PMID 31015840.
  12. ^ Morsczeck, C.; Götz, W.; Schierholz, J.; Zeilhofer, F.; Kühn, U.; Möhl, C.; Sippel, C.; Hoffmann, K. H. (2005). "Isolation of precursor cells (PCS) from human dental follicle of wisdom teeth". Matrix Biology. 24 (2): 155–65. doi:10.1016/j.matbio.2004.12.004. PMID 15890265.
  13. ^ Li, Y.; Yang, Y. Y.; Ren, J. L.; Xu, F.; Chen, F. M.; Li, A. (2017). "Exosomes secreted by stem cells from human exfoliated deciduous teeth contribute to functional recovery after traumatic brain injury by shifting microglia M1/M2 polarization in rats". Stem Cell Research & Therapy. 8 (1): 198. doi:10.1186/s13287-017-0648-5. PMC 5622448. PMID 28962585.
  14. ^ a b c Yao, S.; Tan, L.; Chen, H.; Huang, X.; Zhao, W.; Wang, Y. (2019). "Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker". Stem Cells International. 2019: 3526409. doi:10.1155/2019/3526409. PMC 6431526. PMID 30984268.
  15. ^ a b Xiao, L.; Saiki, C.; Okamura, H. (2019). "Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice". International Journal of Molecular Sciences. 20 (8): 1858. doi:10.3390/ijms20081858. PMC 6514841. PMID 30991705.
  16. ^ Rao, N.; Wang, X.; Zhai, Y.; Li, J.; Xie, J.; Zhao, Y.; Ge, L. (2019). "Stem cells from human exfoliated deciduous teeth ameliorate type II diabetic mellitus in Goto-Kakizaki rats". Diabetology & Metabolic Syndrome. 11: 22. doi:10.1186/s13098-019-0417-y. PMC 6394089. PMID 30858895.
  17. ^ a b c Dai, Y. Y.; Ni, S. Y.; Ma, K.; Ma, Y. S.; Wang, Z. S.; Zhao, X. L. (2019). "Stem cells from human exfoliated deciduous teeth correct the immune imbalance of allergic rhinitis via Treg cells in vivo and in vitro". Stem Cell Research & Therapy. 10 (1): 39. doi:10.1186/s13287-019-1134-z. PMC 6341645. PMID 30670101.
  18. ^ a b c Yokoyama, T.; Yagi Mendoza, H.; Tanaka, T.; Ii, H.; Takano, R.; Yaegaki, K.; Ishikawa, H. (2019). "Regulation of CCL4-induced liver cirrhosis by hepatically differentiated human dental pulp stem cells" (PDF). Human Cell. 32 (2): 125–140. doi:10.1007/s13577-018-00234-0. PMID 30637566. S2CID 58005147.
  19. ^ Mantesso, A.; Sharpe, P. (2009). "Dental stem cells for tooth regeneration and repair". Expert Opinion on Biological Therapy. 9 (9): 1143–54. doi:10.1517/14712590903103795. PMID 19653863. S2CID 10622446.
  20. ^ Miura, M.; Gronthos, S.; Zhao, M.; Lu, B.; Fisher, L. W.; Robey, P. G.; Shi, S. (2003). "SHED: Stem cells from human exfoliated deciduous teeth". Proceedings of the National Academy of Sciences. 100 (10): 5807–12. Bibcode:2003PNAS..100.5807M. doi:10.1073/pnas.0937635100. JSTOR 3147498. PMC 156282. PMID 12716973.
  21. ^ Kerkis, Irina; Kerkis, Alexandre; Dozortsev, Dmitri; Stukart-Parsons, Gaëlle Chopin; Gomes Massironi, Sílvia Maria; Pereira, Lygia V.; Caplan, Arnold I.; Cerruti, Humberto F. (2006). "Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers". Cells Tissues Organs. 184 (3–4): 105–16. doi:10.1159/000099617. PMID 17409736. S2CID 32406588.
  22. ^ Graziano, Antonio; d'Aquino, Riccardo; Angelis, Maria Gabriella Cusella-De; De Francesco, Francesco; Giordano, Antonio; Laino, Gregorio; Piattelli, Adriano; Traini, Tonino; De Rosa, Alfredo; Papaccio, Gianpaolo (2008). "Scaffold's surface geometry significantly affects human stem cell bone tissue engineering". Journal of Cellular Physiology. 214 (1): 166–72. doi:10.1002/jcp.21175. PMID 17565721. S2CID 43642451.
  23. ^ D'aquino, Riccardo; Papaccio, Gianpaolo; Laino, Gregorio; Graziano, Antonio (2008). "Dental Pulp Stem Cells: A Promising Tool for Bone Regeneration". Stem Cell Reviews. 4 (1): 21–6. doi:10.1007/s12015-008-9013-5. PMID 18300003. S2CID 14784845.
  24. ^ Onyekwelu, O; Seppala, M; Zoupa, M; Cobourne, MT (2007). "Tooth development: 2. Regenerating teeth in the laboratory". Dental Update. 34 (1): 20–2, 25–6, 29. doi:10.12968/denu.2007.34.1.20. PMID 17348555.
  25. ^ Cordeiro, Mabel M.; Dong, Zhihong; Kaneko, Tomoatsu; Zhang, Zhaocheng; Miyazawa, Marta; Shi, Songtao; Smith, Anthony J.; Nör, Jacques E. (2008). "Dental Pulp Tissue Engineering with Stem Cells from Exfoliated Deciduous Teeth". Journal of Endodontics. 34 (8): 962–9. doi:10.1016/j.joen.2008.04.009. PMID 18634928.
  26. ^ Gandia, Carolina; Armiñan, Ana; García-Verdugo, Jose Manuel; Lledó, Elisa; Ruiz, Amparo; Miñana, M Dolores; Sanchez-Torrijos, Jorge; Payá, Rafael; Mirabet, Vicente; Carbonell-Uberos, Francisco; Llop, Mauro; Montero, Jose Anastasio; Sepúlveda, Pilar (2008). "Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction". Stem Cells. 26 (3): 638–45. doi:10.1634/stemcells.2007-0484. PMID 18079433. S2CID 9594271.
  27. ^ Kerkis, Irina; Ambrosio, Carlos E; Kerkis, Alexandre; Martins, Daniele S; Zucconi, Eder; Fonseca, Simone AS; Cabral, Rosa M; Maranduba, Carlos MC; Gaiad, Thais P; Morini, Adriana C; Vieira, Natassia M; Brolio, Marina P; Sant'Anna, Osvaldo A; Miglino, Maria A; Zatz, Mayana (2008). "Early transplantation of human immature dental pulp stem cells from baby teeth to golden retriever muscular dystrophy (GRMD) dogs: Local or systemic?". Journal of Translational Medicine. 6: 35. doi:10.1186/1479-5876-6-35. PMC 2529267. PMID 18598348.
  28. ^ Nosrat, I; Widenfalk, J; Olson, L; Nosrat, CA (2001). "Dental Pulp Cells Produce Neurotrophic Factors, Interact with Trigeminal Neurons in Vitro, and Rescue Motoneurons after Spinal Cord Injury". Developmental Biology. 238 (1): 120–32. doi:10.1006/dbio.2001.0400. PMID 11783998.
  29. ^ de Mendonça Costa, André; Bueno, Daniela F.; Martins, Marília T.; Kerkis, Irina; Kerkis, Alexandre; Fanganiello, Roberto D.; Cerruti, Humberto; Alonso, Nivaldo; Passos-Bueno, Maria Rita (2008). "Reconstruction of Large Cranial Defects in Nonimmunosuppressed Experimental Design With Human Dental Pulp Stem Cells". The Journal of Craniofacial Surgery. 19 (1): 204–10. doi:10.1097/scs.0b013e31815c8a54. PMID 18216690. S2CID 23340729.
  30. ^ Vishwakarma, Ajaykumar (13 November 2014). Stem Cell Biology and Tissue Engineering in Dental Sciences. Elsevier. ISBN 978-0-12-397157-9.

Further reading

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