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DeutschFile:Summary of RNA Microarray.svg
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Summary
| Description | English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides and measurement of fluorescent intensity across the array indicates the abundance of a predetermined set of sequences. These sequences are typically specifically chosen to report on genes of interest within the organism’s genome.[1] |
| Date | |
| Source | Own work |
| Author | Thomas Shafee |
| Other versions | Also in series:
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- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:22, 13 February 2017 | | 1,020 × 858 (67 KB) | Evolution and evolvability | text as paths to avoid artefacts |
| 08:40, 10 February 2017 |  | 1,020 × 858 (66 KB) | Evolution and evolvability | Reverted to version as of 05:08, 5 October 2016 (UTC) | |
| 08:40, 10 February 2017 |  | 1,020 × 803 (87 KB) | Evolution and evolvability | reverse order of fragmentation and reverse-transcription. | |
| 05:08, 5 October 2016 |  | 1,020 × 858 (66 KB) | Evolution and evolvability | User created page with UploadWizard |
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