E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18gene.[5][6][7] A knockout in a human colorectal cancer cell line, HCT116, has also been created.[8]
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through interaction with Rad6, which is a ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.[7]
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