Damernas 400 meter häcklöpning vid olympiska sommarspelen 2016, i Rio de Janeiro i Brasilien avgjordes den 15-18 augusti på Estádio Olímpico João Havelange.[1]
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 7 | Tracey, RistanannaRistananna Tracey |  Jamaica | 54,88 | Q |
| 2 | 4 | Hejnová, ZuzanaZuzana Hejnová |  Tjeckien | 55,54 | Q |
| 3 | 1 | Folorunso, AyomideAyomide Folorunso |  Italien | 55,78 | Q |
| 4 | 6 | Troest, StinaStina Troest |  Danmark | 56,06 | q |
| 5 | 2 | McLaughlin, SydneySydney McLaughlin |  USA | 56,32 | q |
| 6 | 3 | Fontanive, PetraPetra Fontanive |  Schweiz | 56,80 | |
| 7 | 8 | Hechavarría, ZurianZurian Hechavarría |  Kuba | 57,28 | |
| 8 | 5 | Maiyo, Maureen JelagatMaureen Jelagat Maiyo |  Kenya | 57,97 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 7 | Linkiewicz, JoannaJoanna Linkiewicz |  Polen | 56,07 | Q |
| 2 | 3 | Russel, JanieveJanieve Russel | Jamaica | 56,13 | Q |
| 3 | 6 | Claxton, GraceGrace Claxton |  Puerto Rico | 56,40 | Q |
| 4 | 8 | Belle, Tia-AdanaTia-Adana Belle |  Barbados | 56,68 | |
| 5 | 2 | McKnight, SparkleSparkle McKnight |  Trinidad och Tobago | 56,80 | |
| 6 | 4 | Baumann, JackieJackie Baumann |  Tyskland | 59,04 | |
| 7 | 5 | Islami, DritaDrita Islami |  Makedonien | 1:01,18 | |
| 8 | 1 | Chase-Taylor, ChaniceChanice Chase-Taylor |  Kanada | 1:02,83 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 5 | Spencer, AshleyAshley Spencer | USA | 55,12 | Q |
| 2 | 7 | Nugent, LeahLeah Nugent | Jamaica | 55,66 | Q |
| 2 | 6 | Tkatjuk, ViktorijaViktorija Tkatjuk |  Ukraina | 56,14 | Q |
| 3 | 8 | Rosolova, DenisaDenisa Rosolova | Tjeckien | 56,36 | q |
| 4 | 1 | Sprunger, LeaLea Sprunger | Schweiz | 56,58 | |
| 5 | 3 | Iuel, AmalieAmalie Iuel |  Norge | 56,75 | |
| 6 | 4 | Lambarki, HayatHayat Lambarki |  Marocko | 1:00,83 | |
| 7 | 2 | Harutjunjan, LilitLilit Harutjunjan |  Armenien | 1:03,13 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 5 | Petersen, SaraSara Petersen | Danmark | 55,20 | Q |
| 2 | 1 | Nel, WendaWenda Nel |  Sydafrika | 55,55 | Q |
| 3 | 4 | Ankiewicz, EmiliaEmilia Ankiewicz | Polen | 55,89 | Q |
| 4 | 7 | Pedroso, YadisleidyYadisleidy Pedroso | Italien | 55,91 | q |
| 5 | 3 | Bellille, JaneilJaneil Bellille | Trinidad och Tobago | 56,25 | q |
| 6 | 2 | Belanovitj, KatsiarynaKatsiaryna Belanovitj |  Belarus | 56,55 | |
| 7 | 8 | Dauwens, AxelleAxelle Dauwens |  Belgien | 57,68 | |
| 8 | 6 | Mohammad, GhofraneGhofrane Mohammad |  Syrien | 58,85 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 3 | Muhammad, DalilahDalilah Muhammad | USA | 55,33 | Q |
| 2 | 7 | Montcalm, NoelleNoelle Montcalm | Kanada | 56,07 | Q |
| 3 | 6 | Titimets, HannaHanna Titimets | Ukraina | 56,24 | Q |
| 4 | 1 | Wells, LaurenLauren Wells |  Australien | 56,26 | q |
| 5 | 2 | Anacharsis, PharaPhara Anacharsis |  Frankrike | 56,64 | |
| 6 | 4 | Barbosa, VeraVera Barbosa |  Portugal | 57,28 | |
| 7 | 5 | Nguyễn, Thị HuyềnThị Huyền Nguyễn |  Vietnam | 57,87 | |
| 8 | 8 | Asanova, NataljaNatalja Asanova |  Uzbekistan | 1:02,37 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 6 | Doyle, EilidhEilidh Doyle |  Storbritannien | 55,46 | Q |
| 2 | 1 | Watson, SageSage Watson | Kanada | 55,93 | Q |
| 3 | 3 | Kolesnjtjenko, OlenaOlena Kolesnjtjenko | Ukraina | 56,61 | Q |
| 4 | 2 | Ogoegbunam, AmakaAmaka Ogoegbunam |  Nigeria | 56,96 | |
| 5 | 5 | Kubokura, SatomiSatomi Kubokura |  Japan | 57,34 | |
| 6 | 8 | Caravelli, MarziaMarzia Caravelli | Italien | 57,77 | |
| 7 | 7 | Scott, SharolynSharolyn Scott |  Costa Rica | 58,27 | |
| 8 | 4 | Romanova, AleksandraAleksandra Romanova |  Kazakstan | 59,36 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 4 | Hejnová, ZuzanaZuzana Hejnová | Tjeckien | 54,55 | Q, SB |
| 2 | 5 | Tracey, RistanannaRistananna Tracey | Jamaica | 54,80 | Q |
| 3 | 6 | Linkiewicz, JoannaJoanna Linkiewicz | Polen | 55,35 | |
| 4 | 1 | Troest, StinaStina Troest | Danmark | 56,00 | SB |
| 5 | 2 | McLaughlin, SydneySydney McLaughlin | USA | 56,22 | |
| 6 | 3 | Montcalm, NoelleNoelle Montcalm | Kanada | 56,28 | |
| 7 | 7 | Folorunso, AyomideAyomide Folorunso | Italien | 56,37 | |
| 8 | 8 | Kolesnjtjenko, OlenaOlena Kolesnjtjenko | Ukraina | 56,77 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 5 | Spencer, AshleyAshley Spencer | USA | 54,87 | Q |
| 2 | 4 | Russel, JanieveJanieve Russel | Jamaica | 54,92 | Q |
| 3 | 3 | Doyle, EilidhEilidh Doyle | Storbritannien | 54,99 | q |
| 4 | 7 | Titimets, HannaHanna Titimets | Ukraina | 55,27 | |
| 5 | 1 | Pedroso, YadisleidyYadisleidy Pedroso | Italien | 55,78 | SB |
| 6 | 6 | Nel, WendaWenda Nel | Sydafrika | 55,83 | |
| 7 | 8 | Ankiewicz, EmiliaEmilia Ankiewicz | Polen | 56,99 | |
| 8 | 2 | Rosolová, DenisaDenisa Rosolová | Tjeckien | 57,39 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
| 1 | 3 | Muhammad, DalilahDalilah Muhammad | USA | 53,89 | Q |
| 2 | 5 | Petersen, SaraSara Petersen | Danmark | 54,55 | Q |
| 3 | 6 | Nugent, LeahLeah Nugent | Jamaica | 54,98 | q, PB |
| 4 | 4 | Watson, SageSage Watson | Kanada | 55,44 | |
| 5 | 7 | Claxton, GraceGrace Claxton | Puerto Rico | 55,85 | PB |
| 6 | 2 | Bellille, JaneilJaneil Bellille | Trinidad och Tobago | 56,06 | SB |
| 7 | 1 | Wells, LaurenLauren Wells | Australien | 56,83 | |
| 8 | 8 | Tkachuk, ViktoriyaViktoriya Tkachuk | Ukraina | 56,87 | |
| Placering | Bana | Idrottare | Nationalitet | Tid | Noteringar |
 | 3 | Muhammad, DalilahDalilah Muhammad | USA | 53,13 | |
 | 4 | Petersen, SaraSara Petersen | Danmark | 53,55 | NR |
 | 5 | Spencer, AshleyAshley Spencer | USA | 53,72 | PB |
| 4 | 6 | Hejnová, ZuzanaZuzana Hejnová | Tjeckien | 53,92 | SB |
| 5 | 7 | Tracey, RistanannaRistananna Tracey | Jamaica | 54,15 | PB |
| 6 | 2 | Nugent, LeahLeah Nugent | Jamaica | 54,45 | PB |
| 7 | 8 | Russel, JanieveJanieve Russel | Jamaica | 54,56 | |
| 8 | 1 | Doyle, EilidhEilidh Doyle | Storbritannien | 54,61 | |
French
Deutsch
Dalilah Muhammad
Sara Petersen
Jamaica
Tjeckien
Italien
Schweiz
Kuba
Kenya
Polen
Puerto Rico
Barbados
Trinidad och Tobago
Tyskland
Makedonien.svg){kind=small}
Kanada
Ukraina
Norge
Marocko
Armenien
Sydafrika
Belarus.svg){kind=small}
Belgien,_Flag_of_Syria_(1980%E2%80%932024).svg){kind=small}
Syrien
Australien
Frankrike
Portugal
Vietnam
Uzbekistan.svg){kind=small}
Storbritannien
Nigeria
Japan
Costa Rica
Kazakstan
